Manual Drainage of the Zebrafish Embryonic Brain Ventricles

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Manual Drainage of the Zebrafish Embryonic Brain Ventricles

DOI:

10.3791/4243-v

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06:17 min

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December 16, 2012

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Jessica T. Chang, Hazel Sive

¹Department of Biology, Whitehead Institute of Biomedical Research,Massachusetts Institute of Technology

Chapters

00:05Title
01:21Preparing Microinjection Needles, Oil Injector Apparatus, and Embryos
02:28Draining and Collecting the Embryonic Cerebrospinal Fluid (eCSF)
04:24Representative eCSF Drainage Results
05:55Conclusion

Summary

자동 번역

We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain ventricular system. This allows for further examination of CSF composition and its requirement during embryonic brain development.

Manual Drainage of the Zebrafish Embryonic Brain Ventricles

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