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Manual Drainage of the Zebrafish Embryonic Brain Ventricles
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December 16, 2012
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We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain ventricular system. This allows for further examination of CSF composition and its requirement during embryonic brain development.
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Manual DrainageZebrafish Embryonic Brain VentriclesCerebrospinal FluidProtein-rich FluidVertebrate Embryonic DevelopmentChoroid PlexusHighly Vascularized Secretory RegionsNeuroepitheliumEmbryonic CSFECSFAdult CSFSecreted FactorsNeurogenesisGene ExpressionCell ProliferationCell SurvivalProteomic Analyses
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Chang, J. T., Sive, H. Manual Drainage of the Zebrafish Embryonic Brain Ventricles. J. Vis. Exp. (70), e4243, doi:10.3791/4243 (2012).
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