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Journal / Neuroscience / 抗体转染神经元作为一种工具来研究疾病的发病机制…
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Neuroscience

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JoVE Journal Neuroscience
抗体转染神经元作为一种工具来研究疾病的发病机制
 
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抗体转染神经元作为一种工具来研究疾病的发病机制

Article DOI: 10.3791/4154-v • 06:56 min • September 26th, 2012
September 26th, 2012 •
1,2,3, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Research Service, Veterans Administration Medical Center, Memphis, TN, 2Department of Neurology, University of Tennessee Health Science Center, Memphis, TN, 3Department of Anatomy/Neurobiology, University of Tennessee Health Science Center, Memphis, TN

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一种快速的方法，调查相关的抗体的存在下，在细胞内环境中的分子机制的相互作用和效果进行说明。该方法涉及的抗体的转染到活细胞，使用的基础上的脂质制剂的非共价复合物的形成。该技术是适应的永生化细胞系和原代细胞。

Tags

第67期神经科学，医学，分子生物学，免疫学，转染，抗体，神经细胞，免疫细胞化学，荧光显微镜，自身免疫性疾病
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