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Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
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Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
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July 23, 2012
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Microglia are resident macrophages that provide the first line of defense and immune surveillance of the central nervous system. MicroRNAs are regulatory molecules that play an important role in many physiological processes including activation and differentiation of macrophages. In this article, we describe the method for measurement of microRNAs in microglia.
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Veremeyko, T., Starossom, S., Weiner, H. L., Ponomarev, E. D. Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation. J. Vis. Exp. (65), e4097, doi:10.3791/4097 (2012).
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