Journal
/
Imunologia e Infecção
/
Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

DOI:
10.3791/4097-v

•

13:36 min

•

July 23, 2012

•

, , ,
1Center for Neurologic Diseases, Brigham and Women’s Hospital,Harvard Medical School

Capítulos

  • 00:05Título
  • 01:34Isolation of Microglia from Normal CNS
  • 03:04Control of Purity of Microglia by Flow Cytometry
  • 04:27Isolation of Microglia and Peripheral Macrophages from Inflamed CNS in Mouse Model of Neuroinflamation
  • 05:43Isolation of RNA
  • 07:43Normalization and Data Analysis
  • 10:13Results: Analysis of miR-124 Expression in Microglia and Macrophages
  • 13:14Conclusion

Summary

Tadução automática

Tadução automática

Microglia are resident macrophages that provide the first line of defense and immune surveillance of the central nervous system. MicroRNAs are regulatory molecules that play an important role in many physiological processes including activation and differentiation of macrophages. In this article, we describe the method for measurement of microRNAs in microglia.

Tags

MicroRNAsMicrogliaReal-time PCRNeuroinflammationCNSCD11bCD45Activation MarkersPathological EventsPhenotypeFunctionsMiRNAsCell GrowthDifferentiationInflammationInnate Immune CellsMacrophagesMRNAExpression Analysis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Culturing Microglia from the Neonatal and Adult Central Nervous System

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Read Article