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Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Chapters
- 00:00Title
- 01:35Crystallisation Plate Preparation
- 03:54Robot I: In Meso Robot
- 08:48Priming the Syringe
- 09:27Running the Robot
- 11:14Sealing the Plate
- 13:13Robot 2: Mosquito LCP
- 18:00Robot 3: Gryphon LCP
- 19:55Concluding Remarks
Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced.
Tags
RobotHigh-throughput CrystallizationMembrane ProteinsLipidic MesophasesMacromolecular X-ray CrystallographyMXDiffraction-quality CrystalsIn Meso MethodLipidic Cubic Phase (LCP) MethodBicontinuous MesophaseToothpaste-like ConsistencyDispensingReproducible MannerSmall VolumesCrystallization WellsManual ApproachSpecialty ApplicationsCrystal OptimizationDerivatization
