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Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
JoVE Journal
生物学
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JoVE Journal 生物学
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

DOI:

20:21 min

September 01, 2012

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  • 00:00標題
  • 01:35Crystallisation Plate Preparation
  • 03:54Robot I: In Meso Robot
  • 08:48Priming the Syringe
  • 09:27Running the Robot
  • 11:14Sealing the Plate
  • 13:13Robot 2: Mosquito LCP
  • 18:00Robot 3: Gryphon LCP
  • 19:55Concluding Remarks

概要

自動翻訳

Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced.

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