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Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
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September 01, 2012
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Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced.
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RobotHigh-throughput CrystallizationMembrane ProteinsLipidic MesophasesMacromolecular X-ray CrystallographyMXDiffraction-quality CrystalsIn Meso MethodLipidic Cubic Phase (LCP) MethodBicontinuous MesophaseToothpaste-like ConsistencyDispensingReproducible MannerSmall VolumesCrystallization WellsManual ApproachSpecialty ApplicationsCrystal OptimizationDerivatization
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Li, D., Boland, C., Walsh, K., Caffrey, M. Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases. J. Vis. Exp. (67), e4000, doi:10.3791/4000 (2012).
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