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Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
 

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Article DOI: 10.3791/3928-v • 11:39 min • September 21st, 2012
September 21st, 2012 •
1, 1, 2, 1
1Department of Cellular and Molecular Medicine, University of California, San Diego, 2Biosecurity and Public Health, Los Alamos National Laboratory

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요약
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Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

Tags

Unfixed Tissues Frozen Tissues Natural Mucin Distribution Mucins Glycoproteins Mucus Layer Host Innate Immunity Antimicrobial Peptides Cytokines Immunoglobulins Histological Methods Tissue Collection Staining Formalin Fixation Paraffin Embedding Tissue Freezing Cryo-protectant Media
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