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Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
JoVE Journal
Biologia
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JoVE Journal Biologia
Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

47,294 Views

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11:39 min

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September 21, 2012

DOI:

10.3791/3928-v

DOI:
10.3791/3928-v

•

11:39 min
September 21, 2012

•

10 Views
, , ,
1Department of Cellular and Molecular Medicine,University of California, San Diego , 2Biosecurity and Public Health,Los Alamos National Laboratory

Summary

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Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

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Cohen, M., Varki, N. M., Jankowski, M. D., Gagneux, P. Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution. J. Vis. Exp. (67), e3928, doi:10.3791/3928 (2012).

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