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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Chapters
- 00:05Title
- 03:07FV 1-Stage Microplate Activity Assay
- 09:27FV 2-Stage Microplate Activity Assay
- 11:31FV Activity in Normal Human Plasma and in Patients With Disseminated Intravascular Coagulation (DIC)
- 12:15Conclusion
This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.
Tags
Factor V ActivityHuman PlasmaMicroplate Coagulation AssayBlood CoagulationThrombinFibrinogenClot FormationProthrombinaseFV AssaysAutomated FV AssaysMicroplate PlatformEnzyme-catalyzed EventsClot LysisPlatelet AggregationCoagulation FactorsFV Activity MeasurementKinetic Microplate ReaderAbsorbance Change At 405nm
