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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

18,786 Views

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13:08 min

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September 09, 2012

DOI:

10.3791/3822-v

DOI:
10.3791/3822-v

•

13:08 min
September 09, 2012

•

7 Views
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1Applied Bioscience Program, Faculty of Science,University of Ontario Institute of Technology , 2Nursing Program, Faculty of Health Sciences,University of Ontario Institute of Technology , 3Medical Laboratory Science Program, Faculty of Health Sciences,University of Ontario Institute of Technology

Summary

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This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.

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Factor V ActivityHuman PlasmaMicroplate Coagulation AssayBlood CoagulationThrombinFibrinogenClot FormationProthrombinaseFV AssaysAutomated FV AssaysMicroplate PlatformEnzyme-catalyzed EventsClot LysisPlatelet AggregationCoagulation FactorsFV Activity MeasurementKinetic Microplate ReaderAbsorbance Change At 405nm

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Tilley, D., Levit, I., Samis, J. A. Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay. J. Vis. Exp. (67), e3822, doi:10.3791/3822 (2012).

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