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Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
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Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
Chapters
- 00:05Title
- 01:28Preparing CFA-emulsified Antigen
- 03:08Immunization of Donor Mice
- 03:51Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
- 06:07Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
- 06:47Antigenic Challenge of Recipient Mice and Development of Disease
- 07:31Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
- 07:58Conclusion
Certain mouse strains are able to resist induction of experimental autoimmune encephalomyelitis (EAE) with myelin basic protein. Described here is a simple immunization protocol that reverses the unresponsiveness and induces paralytic disease in several typical EAE resistant mouse stains.
Tags
Experimental Autoimmune EncephalomyelitisEAEMouse StrainsAdoptive TransferAntigenic ChallengeCentral Nervous SystemMononuclear CellsParalytic DiseaseMultiple SclerosisGenetic ControlC57BL/6 MiceMyelin Basic ProteinAntigen ProcessingAntigen PresentationEncephalitogenic T Cell ClonesCellular Adoptive Transfer System
