Journal
/
Imunologia e Infecção
/
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

DOI:
10.3791/3778-v

•

08:38 min

•

April 09, 2012

•

, ,
1Department of Medicine, Section of Cardiology,St. John-Providence Health System, 2Department of Immunology and Microbiology,Wayne State University School of Medicine

Capítulos

  • 00:05Título
  • 01:28Preparing CFA-emulsified Antigen
  • 03:08Immunization of Donor Mice
  • 03:51Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
  • 06:07Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
  • 06:47Antigenic Challenge of Recipient Mice and Development of Disease
  • 07:31Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
  • 07:58Conclusion

Summary

Tadução automática

Tadução automática

Certain mouse strains are able to resist induction of experimental autoimmune encephalomyelitis (EAE) with myelin basic protein. Described here is a simple immunization protocol that reverses the unresponsiveness and induces paralytic disease in several typical EAE resistant mouse stains.

Tags

Experimental Autoimmune EncephalomyelitisEAEMouse StrainsAdoptive TransferAntigenic ChallengeCentral Nervous SystemMononuclear CellsParalytic DiseaseMultiple SclerosisGenetic ControlC57BL/6 MiceMyelin Basic ProteinAntigen ProcessingAntigen PresentationEncephalitogenic T Cell ClonesCellular Adoptive Transfer System

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Read Article