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Chapters
- 00:05Title
- 01:40Surgery Preparation and Animal Mounting
- 04:02The Generation of a Polished and Reinforced Thinned-skull Window
- 07:14Imaging Preparation
- 08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
- 09:30Conclusion
Se presenta un método para formar una ventana de imagen en el cráneo del ratón que se extiende milímetros y es estable durante meses sin inflamación del cerebro. Este método es muy adecuado para estudios longitudinales de flujo de sangre, la dinámica celular, y células / estructura vascular utilizando microscopía de dos fotones.
Tags
Polished And Reinforced Thinned-skull WindowLong-term ImagingMouse BrainIn Vivo ImagingCortical FunctionOptical AccessIntracranial EnvironmentPoRTS WindowOptical ClarityBone Regrowth InhibitionLight Scattering ReductionInflammation ReductionTwo-photon Laser Scanning MicroscopyCerebral Blood FlowCellular FunctionAnesthetized PreparationsAwake PreparationsOptogeneticsBlood Flow Disruption
