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Chapters
- 00:05Title
- 01:40Surgery Preparation and Animal Mounting
- 04:02The Generation of a Polished and Reinforced Thinned-skull Window
- 07:14Imaging Preparation
- 08:20Representative Results for Long-term Imaging and optical Manipulation of the Mouse Cortex
- 09:30Conclusion
We presenteren een methode om een beeldvorming raam in de muis schedel die millimeter overspant en is stabiel gedurende maanden zonder ontsteking van de hersenen vormen. Deze methode is zeer geschikt voor longitudinale studies van de bloedstroom, cellulaire dynamiek en cel / vasculaire structuur met behulp van twee-foton microscopie.
Tags
Polished And Reinforced Thinned-skull WindowLong-term ImagingMouse BrainIn Vivo ImagingCortical FunctionOptical AccessIntracranial EnvironmentPoRTS WindowOptical ClarityBone Regrowth InhibitionLight Scattering ReductionInflammation ReductionTwo-photon Laser Scanning MicroscopyCerebral Blood FlowCellular FunctionAnesthetized PreparationsAwake PreparationsOptogeneticsBlood Flow Disruption
