/
/
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Chapters
- 00:05Title
- 01:45Isolation, Stimulation, and Labeling of Immune Cells
- 03:48ImageStream Analysis
- 05:59Results: Representative Images of NFkB Translocation in Specific mDCs-lineage Cell Groups in Response to TLR Signaling
- 07:13Conclusion
We describe use of ImageStream technology (www.amnis.com), which combines quantitative flow cytometry with simultaneous high-resolution digital imaging, to quantify cellular mechanisms of primary immune cells from well-defined patient cohorts. Our studies provide a blueprint for translational investigations to quantify lineage specific cellular responses in small samples from subject cohorts.
Tags
Quantitative ImagingLineage-specificToll-like Receptor-mediated SignalingMonocytesDendritic CellsSmall SamplesHuman BloodImmune StatusInfectionDisease SeverityOutcomeAgingViral InfectionsBacterial InfectionsVaccinesHumoral Immune ResponsesCell-mediated Immune ResponsesToll-like Receptors (TLRs)Innate Immune SystemAntimicrobial Host Defense ResponsesPeripheral Blood MonocytesElderly DonorsDendritic Cells (DCs)Antigen-presenting CellsInnate ImmunityAdaptive ImmunityDysregulation Of TLR3MacrophagesIFN ProductionWest Nile Virus InfectionImmunosenescenceTherapeutic Intervention
