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Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
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April 16, 2012
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We describe use of ImageStream technology (www.amnis.com), which combines quantitative flow cytometry with simultaneous high-resolution digital imaging, to quantify cellular mechanisms of primary immune cells from well-defined patient cohorts. Our studies provide a blueprint for translational investigations to quantify lineage specific cellular responses in small samples from subject cohorts.
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Quantitative ImagingLineage-specificToll-like Receptor-mediated SignalingMonocytesDendritic CellsSmall SamplesHuman BloodImmune StatusInfectionDisease SeverityOutcomeAgingViral InfectionsBacterial InfectionsVaccinesHumoral Immune ResponsesCell-mediated Immune ResponsesToll-like Receptors (TLRs)Innate Immune SystemAntimicrobial Host Defense ResponsesPeripheral Blood MonocytesElderly DonorsDendritic Cells (DCs)Antigen-presenting CellsInnate ImmunityAdaptive ImmunityDysregulation Of TLR3MacrophagesIFN ProductionWest Nile Virus InfectionImmunosenescenceTherapeutic Intervention
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Qian, F., Montgomery, R. R. Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood. J. Vis. Exp. (62), e3741, doi:10.3791/3741 (2012).
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