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Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays
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March 23, 2012
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Explants from the midbrain dopamine system and striatum are used in a collagen matrix assay for the in vitro analysis of mesostriatal and striatonigral pathway development. In this assay axonal outgrowth and guidance can be manipulated and quantified. It can also be modified for assessing other regions or molecular cues.
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DissectionCultureMouseDopaminergicStriatalExplantsThree-dimensional Collagen Matrix AssaysMidbrain Dopamine NeuronsStriatumAxon TractsAxon GrowthGuidance CuesSoluble FactorsIn VitroCollagen MatrixStable GradientsProteins ReleasedRat Tail Collagen PurificationMicrodissectionDopaminergic ExplantsStriatal ExplantsCollagen GelsImmunohistochemical AnalysisQuantitative AnalysisAxonal Projections
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Schmidt, E. R., Morello, F., Pasterkamp, R. J. Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays. J. Vis. Exp. (61), e3691, doi:10.3791/3691 (2012).
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