/
/
Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays
25,442 Views
•
08:24 min
•
March 23, 2012
DOI:
Summary
Automatically generated
Explants from the midbrain dopamine system and striatum are used in a collagen matrix assay for the in vitro analysis of mesostriatal and striatonigral pathway development. In this assay axonal outgrowth and guidance can be manipulated and quantified. It can also be modified for assessing other regions or molecular cues.
Explore More Videos
DissectionCultureMouseDopaminergicStriatalExplantsThree-dimensional Collagen Matrix AssaysMidbrain Dopamine NeuronsStriatumAxon TractsAxon GrowthGuidance CuesSoluble FactorsIn VitroCollagen MatrixStable GradientsProteins ReleasedRat Tail Collagen PurificationMicrodissectionDopaminergic ExplantsStriatal ExplantsCollagen GelsImmunohistochemical AnalysisQuantitative AnalysisAxonal Projections
Read Article
Cite this Article
Schmidt, E. R., Morello, F., Pasterkamp, R. J. Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays. J. Vis. Exp. (61), e3691, doi:10.3791/3691 (2012).
Copy