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Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
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Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
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April 23, 2012
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Here we describe a protocol based only on cell infection, which improves the efficiency of recombinant parvovirus production by more than 100 fold in comparison to other protocols in use. This protocol relies on the use of a novel adenovirus 5-based helper containing the parvovirus VP transcription unit (Ad-VP).
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El-Andaloussi, N., Leuchs, B., Bonifati, S., Rommelaere, J., Marchini, A. Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems. J. Vis. Exp. (62), e3518, doi:10.3791/3518 (2012).
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