Journal
/
생물학
/
Analysis of Cell Cycle Position in Mammalian Cells
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

DOI:
10.3791/3491-v

•

12:19 min

•

January 21, 2012

•

, ,
1Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario, 2London Regional Cancer Program, Children’s Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario

Chapters

  • 00:05Title
  • 01:19Labeling and Fixing of Cells
  • 02:30Denaturing and Staining of Brdu and DNA
  • 04:11Flow Cytometry and Data Analysis
  • 06:10Alternate Protocol for Cell Cycle Analysis in a Mixed Population of Cells
  • 07:35Cell Cycle Distribution Staining Can Be Used for a Variety of Experimental Purposes
  • 11:47Conclusion

Summary

자동 번역

자동 번역

Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.

Tags

Cell Cycle PositionMammalian CellsCell ProliferationCell Cycle PhasesDNA SynthesisGrowth RegulationPropidium IodideFlow CytometryRadioactive ThymidineBromodeoxyuridineFluorodeoxyuridineBrdU Staining

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Expression Analysis of Mammalian Linker-histone Subtypes

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Induction and Analysis of Epithelial to Mesenchymal Transition

Measurement of Heme Synthesis Levels in Mammalian Cells

Read Article