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Analysis of Cell Cycle Position in Mammalian Cells
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JoVE Revista Biología
Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

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12:19 min

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January 21, 2012

DOI:

10.3791/3491-v

DOI:
10.3791/3491-v

•

12:19 min
January 21, 2012

•

29 Views
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1Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario, 2London Regional Cancer Program, Children’s Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario

Summary

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Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.

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Cite this Article

Cecchini, M. J., Amiri, M., Dick, F. A. Analysis of Cell Cycle Position in Mammalian Cells. J. Vis. Exp. (59), e3491, doi:10.3791/3491 (2012).

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