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Immunology and Infection

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JoVE Journal Immunology and Infection
A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)
 
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A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)

Article DOI: 10.3791/3332-v • 08:11 min • September 14th, 2011
September 14th, 2011 •
1, 2, 1
1Department of Paediatrics, Imperial College London, 2Centre for Health Sciences, Barts & The London School of Medicine and Dentistry

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We describe an alternative approach to the enumeration of mycobacteria in vitro, which uses reporter-gene tagged mycobacteria instead of colony-forming units (CFU). “Survival” of organisms as well as host response-markers are measured simultaneously, providing a low-cost, versatile and functional system for studies of host/pathogen interactions in the context of tuberculosis.

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Whole Blood Assay Viability Measurement Mycobacteria Reporter-gene Tagged BCG Reporter-gene Tagged M.Tb Functional Assays Immunogenicity Measurement Serum Bactericidal Titers Childhood Vaccines Cut-off Levels Protective Immune Responses Cell-mediated Immunity Effector T Cell Responses TB Vaccines Animal Model Evaluation Novel TB-vaccine Candidates Phase 1 Human Trials Assessment Of Immunogenicity Protective Efficacy Anti-TB Vaccines Bactericidal Assays Tuberculosis Vaccine Research
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