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A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)
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A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)
Chapters
- 00:05Title
- 01:35Preparation of BCG lux Reporter Mycobacterial Stock
- 02:34RLU/CFU Correlation
- 03:43Whole Blood Assay
- 06:08Growth Ratios as Functional Responses
- 07:20Conclusion
We describe an alternative approach to the enumeration of mycobacteria in vitro, which uses reporter-gene tagged mycobacteria instead of colony-forming units (CFU). “Survival” of organisms as well as host response-markers are measured simultaneously, providing a low-cost, versatile and functional system for studies of host/pathogen interactions in the context of tuberculosis.
Tags
Whole Blood AssayViability MeasurementMycobacteriaReporter-gene Tagged BCGReporter-gene Tagged M.TbFunctional AssaysImmunogenicity MeasurementSerum Bactericidal TitersChildhood VaccinesCut-off LevelsProtective Immune ResponsesCell-mediated ImmunityEffector T Cell ResponsesTB VaccinesAnimal Model EvaluationNovel TB-vaccine CandidatesPhase 1 Human TrialsAssessment Of ImmunogenicityProtective EfficacyAnti-TB VaccinesBactericidal AssaysTuberculosis Vaccine Research
