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Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
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신경과학
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JoVE 신문 신경과학
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

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10:47 min

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October 28, 2011

DOI:

10.3791/3273-v

DOI:
10.3791/3273-v

•

10:47 min
October 28, 2011

•

8 Views
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1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery,Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, 6La Jolla IVF

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We have established a protocol for induction of neuroblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human neuronal progenitors and neuronal cell types in the developing CNS for neural repair.

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Human Neuronal ProgenitorsNeuronsPluripotent Human Embryonic Stem CellsSmall Molecule InductionCell-based TherapiesCentral Nervous System (CNS)Neural Stem CellsExpansion AbilityPlasticityTrophic And Neuroprotective MoleculesPluripotent HESCsNeurological DisordersDifferentiation PotentialDevelopmental StudyClinical TranslationMulti-lineage InclinationGerm Layer

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Parsons, X. H., Teng, Y. D., Parsons, J. F., Snyder, E. Y., Smotrich, D. B., Moore, D. A. Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction. J. Vis. Exp. (56), e3273, doi:10.3791/3273 (2011).

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