This content is Free Access.
A Protocol for Computer-Based Protein Structure and Function Prediction
67,701 Views
•
16:41 min
•
November 03, 2011
DOI:
Summary
Automatically generated
Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.
Explore More Videos
ProtocolComputer-basedProtein StructureFunction PredictionGenome Sequencing ProjectsProtein SequenceStructure And Function KnowledgeExperimental MethodsComputational ModelingI-TASSER ServerSecondary Structure PredictionSolvent Accessibility PredictionHomologous Template ProteinsStructure AlignmentsTertiary Structural ModelsStructure-based Functional AnnotationsConfidence ScoreEnd UsersUser-specified Inter-residue DistanceContact MapsI-TASSER ModelingTemplate ProteinsStructure Assembly Simulations
Read Article
Cite this Article
Roy, A., Xu, D., Poisson, J., Zhang, Y. A Protocol for Computer-Based Protein Structure and Function Prediction. J. Vis. Exp. (57), e3259, doi:10.3791/3259 (2011).
Copy