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A Protocol for Computer-Based Protein Structure and Function Prediction
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Biología
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JoVE Revista Biología
A Protocol for Computer-Based Protein Structure and Function Prediction

A Protocol for Computer-Based Protein Structure and Function Prediction

67,701 Views

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16:41 min

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November 03, 2011

DOI:

10.3791/3259-v

DOI:
10.3791/3259-v

•

16:41 min
November 03, 2011

•

33 Views
, , ,
1Center for Computational Medicine and Bioinformatics,University of Michigan , 2Center for Bioinformatics and Department of Molecular Bioscience,University of Kansas

Summary

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Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.

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ProtocolComputer-basedProtein StructureFunction PredictionGenome Sequencing ProjectsProtein SequenceStructure And Function KnowledgeExperimental MethodsComputational ModelingI-TASSER ServerSecondary Structure PredictionSolvent Accessibility PredictionHomologous Template ProteinsStructure AlignmentsTertiary Structural ModelsStructure-based Functional AnnotationsConfidence ScoreEnd UsersUser-specified Inter-residue DistanceContact MapsI-TASSER ModelingTemplate ProteinsStructure Assembly Simulations

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Cite this Article

Roy, A., Xu, D., Poisson, J., Zhang, Y. A Protocol for Computer-Based Protein Structure and Function Prediction. J. Vis. Exp. (57), e3259, doi:10.3791/3259 (2011).

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