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A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
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A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

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14:39 min

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November 28, 2011

DOI:

10.3791/3148-v

DOI:
10.3791/3148-v

•

14:39 min
November 28, 2011

•

1 Views
, , , , , ,
1Department of Biotechnology & Bioinformatics,California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism,Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Summary

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A three-dimensional clonogenic assay that allows pancreatic-like progenitors to differentiate into insulin-expressing colonies is described. This method takes advantage of semi-solid media containing methylcellulose, Matrigel and growth factors, in which single progenitors proliferate and differentiate in vitro, permitting quantification of the number of functional progenitors in a population.

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Cite this Article

Winkler, M., Trieu, N., Feng, T., Jin, L., Walker, S., Singh, L., Ku, H. T. A Quantitative Assay for Insulin-expressing Colony-forming Progenitors. J. Vis. Exp. (57), e3148, doi:10.3791/3148 (2011).

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