Journal
/
生物学
/
A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

13,243 Views

•

14:39 min

•

November 28, 2011

DOI:

10.3791/3148-v

DOI:
10.3791/3148-v

•

14:39 min
November 28, 2011

•

1 Views
, , , , , ,
1Department of Biotechnology & Bioinformatics,California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism,Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Summary

Automatically generated

A three-dimensional clonogenic assay that allows pancreatic-like progenitors to differentiate into insulin-expressing colonies is described. This method takes advantage of semi-solid media containing methylcellulose, Matrigel and growth factors, in which single progenitors proliferate and differentiate in vitro, permitting quantification of the number of functional progenitors in a population.

Explore More Videos

Quantitative AssayInsulin-expressing Colony-forming ProgenitorsPancreatic Stem CellsProgenitor Cell BiologyIn Vitro AssaySingle Cell AnalysisLineage PotentialPancreatospheresSuspension CultureSingle Cell DepositionCulture MediaNumerationLabor-intensivePancreatosphere-initiating ProgenitorsProliferation And DifferentiationSemi-solid Media Based Colony AssayMethylcelluloseThree-dimensional Space

Read Article

Cite this Article

Winkler, M., Trieu, N., Feng, T., Jin, L., Walker, S., Singh, L., Ku, H. T. A Quantitative Assay for Insulin-expressing Colony-forming Progenitors. J. Vis. Exp. (57), e3148, doi:10.3791/3148 (2011).

Download .ris file

Copy

Share Video

.

Copy