JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
13,424 Views
•
07:09 min
•
September 08, 2011
DOI:
Summary
Automatically generated
We describe procedures for repeated administration of inhibitors of muscarinic signaling to the levator auris longus (LAL) muscle of young adult mice and for subsequent immunostaining of its neuromuscular junctions (NMJs) in wholemounts. The LAL muscle has unique advantages for revealing in vivo pharmacological effects on NMJs.
Explore More Videos
Subcutaneous AdministrationMuscarinic AntagonistsTriple-immunostainingLevator Auris Longus MuscleMiceHind Limb MusclesIn Vivo Pharmacological StudiesNMJsDrug PenetrationSubcutaneous AdministrationIntramuscular AdministrationSystemic AdministrationMini-pumpsInflammatory ResponsesDirect Muscle DamageHind Limb Muscle AnalysisSerial SectioningExtensive ImmunostainingMouse LAL MusclePinna MovementFacial Nerve BranchStylomastoid Foramen
Read Article
Cite this Article
Wright, M., Kim, A., Son, Y. Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice. J. Vis. Exp. (55), e3124, doi:10.3791/3124 (2011).
Copy