/
/
Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,424 Views
•
07:09 min
•
September 08, 2011
DOI:
Summary
Automatically generated
We describe procedures for repeated administration of inhibitors of muscarinic signaling to the levator auris longus (LAL) muscle of young adult mice and for subsequent immunostaining of its neuromuscular junctions (NMJs) in wholemounts. The LAL muscle has unique advantages for revealing in vivo pharmacological effects on NMJs.
Explore More Videos
Subcutaneous AdministrationMuscarinic AntagonistsTriple-immunostainingLevator Auris Longus MuscleMiceHind Limb MusclesIn Vivo Pharmacological StudiesNMJsDrug PenetrationSubcutaneous AdministrationIntramuscular AdministrationSystemic AdministrationMini-pumpsInflammatory ResponsesDirect Muscle DamageHind Limb Muscle AnalysisSerial SectioningExtensive ImmunostainingMouse LAL MusclePinna MovementFacial Nerve BranchStylomastoid Foramen
Read Article
Cite this Article
Wright, M., Kim, A., Son, Y. Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice. J. Vis. Exp. (55), e3124, doi:10.3791/3124 (2011).
Copy