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Isolation of Functional Cardiac Immune Cells
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December 05, 2011
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Summary
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This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
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Functional Cardiac Immune CellsPathological RemodelingCardiac DiseasesMast CellsT-cellsMacrophagesBiologically Active MediatorsCytokinesProteasesTryptaseExtracellular Matrix MetabolismMatrix MetalloproteinasesCollagen AccumulationCardiac Fibroblasts’ FunctionIsolating Cardiac Immune CellsLoss Of FunctionTechnique DevelopmentUnderstanding Of Role In Cardiac DiseaseAnatomical LocationRat’s Xiphoid ProcessAxillaFalciform LigamentPericardium Of The HeartCharacterization Of FunctionalityPhenotypeMaturity
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McLarty, J. L., Meléndez, G. C., Spencer, W. J., Levick, S. P., Brower, G. L., Janicki, J. S. Isolation of Functional Cardiac Immune Cells. J. Vis. Exp. (58), e3020, doi:10.3791/3020 (2011).
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