このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Isolation of Functional Cardiac Immune Cells
14,779 Views
•
07:26 min
•
December 05, 2011
DOI:
概要
Automatically generated
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
Explore More Videos
Functional Cardiac Immune CellsPathological RemodelingCardiac DiseasesMast CellsT-cellsMacrophagesBiologically Active MediatorsCytokinesProteasesTryptaseExtracellular Matrix MetabolismMatrix MetalloproteinasesCollagen AccumulationCardiac Fibroblasts’ FunctionIsolating Cardiac Immune CellsLoss Of FunctionTechnique DevelopmentUnderstanding Of Role In Cardiac DiseaseAnatomical LocationRat’s Xiphoid ProcessAxillaFalciform LigamentPericardium Of The HeartCharacterization Of FunctionalityPhenotypeMaturity
Read Article
Cite this Article
McLarty, J. L., Meléndez, G. C., Spencer, W. J., Levick, S. P., Brower, G. L., Janicki, J. S. Isolation of Functional Cardiac Immune Cells. J. Vis. Exp. (58), e3020, doi:10.3791/3020 (2011).
Copy