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Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice
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09:45 min
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July 27, 2011
DOI:
1Department of Medicine,Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program,Memorial Sloan Kettering Cancer Center, 4Howard Hughes,Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics,The Rockefeller University
Summary
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The interstitial cells of Cajal (ICC) are the pacemaker cells of the gastrointestinal (GI) tract. They form complex networks between smooth muscle cells and post-ganglionic neuronal fibers to regulate GI contractility. Here, we present immunofluorescence methods cross-sectional and whole-mount visualization of murine ICC networks.
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Chen, Y., Shamu, T., Chen, H., Besmer, P., Sawyers, C. L., Chi, P. Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice. J. Vis. Exp. (53), e2802, doi:10.3791/2802 (2011).
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