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制备和使用艾滋病毒感染主的CD4 + T细胞作为靶细胞,自然杀伤细胞的细胞毒性检测
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
DOI:

12:07 min

March 14, 2011

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Chapters

  • 00:05Title
  • 01:14Infection of CD4+ T-Cells with HIV-1
  • 02:42Purification of HIV-1 Infected Cells
  • 03:41CD107a Degranulation Assay
  • 06:4151Cr Release Assay
  • 10:07Evaluation of CD107a Degranulation and NK Cell Cytotoxicity
  • 11:45Conclusion

Summary

자동 번역

细胞毒性实验来衡量HIV感染的细胞自然杀伤细胞裂解反应是有限的靶细胞的纯度。我们这里展示的高度纯化的HIV - 1感染的主要T细胞爆炸的人口利用HIV - 1病毒的能力,向下调整的CD4隔离。

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