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Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

25,614 Views

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12:07 min

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March 14, 2011

DOI:

10.3791/2668-v

DOI:
10.3791/2668-v

•

12:07 min
March 14, 2011

•

12 Views
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1Department of Immunology and Microbiology,Rush University Medical Center

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Cytotoxicity assays to measure natural killer cell lytic responses to HIV-infected cells is limited by the purity of the target cells. We demonstrate here the isolation of a highly purified population of HIV-1 infected primary T-cell blasts by taking advantage of HIV-1 s ability to down-modulate CD4.

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HIV-1Primary CD4+ T-cellsNatural Killer CellsCytotoxic AssaysTarget CellsNK Cell FunctionCytolytic ActivityHIV-infected CellsT-cell BlastsInfected-T-cell LinesAutologous Primary CellsMajor Histocompatibility Complex Class I MismatchesNK Cell LysisInfected Cell To Uninfected Cell Ratio

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Davis, Z. B., Ward, J. P., Barker, E. Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays. J. Vis. Exp. (49), e2668, doi:10.3791/2668 (2011).

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