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Organotypic Culture of Full-thickness Adult Porcine Retina
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08:17 min
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March 20, 2011
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Here we describe a cost-effective technique for organotypic culture of adult porcine retina for seven days. Briefly, a sterile filter paper was used to lift the neural retina off from the RPE and place photoreceptor side up on an insert raised by a custom-made stand.
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Organotypic CultureFull-thicknessAdult Porcine RetinaIn Vitro ModelsAnimal ExperimentsHuman Retinal InjuryDegenerative DiseaseCost-effective TechniqueAbattoirTrephine BladeNeural RetinaBruch’s MembraneRPE-choroid-sclera ExplantsSterile Filter PaperPhotoreceptor Side UpInsertCustom-made StandCulture MediumNative Retinal StructureMorphological StudiesPharmacological StudiesBiochemical Studies
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Wang, J., Kolomeyer, A. M., Zarbin, M. A., Townes-Anderson, E. Organotypic Culture of Full-thickness Adult Porcine Retina. J. Vis. Exp. (49), e2655, doi:10.3791/2655 (2011).
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