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Organotypic Culture of Full-thickness Adult Porcine Retina
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Neurosciences
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Journal JoVE Neurosciences
Organotypic Culture of Full-thickness Adult Porcine Retina

Organotypic Culture of Full-thickness Adult Porcine Retina

DOI:
10.3791/2655-v

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08:17 min

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March 20, 2011

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, , ,
1Neurology and Neurosciences,University of Medicine and Dentistry of New Jersey – UMDNJ, 2Institute of Ophthalmology and Visual Science,University of Medicine and Dentistry of New Jersey – UMDNJ

Chapitres

  • 00:01Titre
  • 00:50Make Custom Stand
  • 01:28Prepare Filter Papers for Culture
  • 01:37Isolate Retina from Pig Eye
  • 04:28Culturing of Isolated Retina
  • 06:03Sectioning of Retina
  • 07:31Immunohistochemistry
  • 07:58Discussion

Summary

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Here we describe a cost-effective technique for organotypic culture of adult porcine retina for seven days. Briefly, a sterile filter paper was used to lift the neural retina off from the RPE and place photoreceptor side up on an insert raised by a custom-made stand.

Tags

Organotypic CultureFull-thicknessAdult Porcine RetinaIn Vitro ModelsAnimal ExperimentsHuman Retinal InjuryDegenerative DiseaseCost-effective TechniqueAbattoirTrephine BladeNeural RetinaBruch’s MembraneRPE-choroid-sclera ExplantsSterile Filter PaperPhotoreceptor Side UpInsertCustom-made StandCulture MediumNative Retinal StructureMorphological StudiesPharmacological StudiesBiochemical Studies

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