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Estabelecimento e propagação de Tumores Humanos em Retinoblastoma Imune camundongos deficientes
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August 04, 2011
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1Interdepartmental Program in Translational Biology & Molecular Medicine,Baylor College of Medicine, 2Texas Children's Cancer Center,Baylor College of Medicine, 3Department of Pediatrics,Baylor College of Medicine, 4Department of Pathology,The Methodist Hospital Research Institute, 5Department of Ophthalmology,Retinoblastoma Center of Houston, 6Baylor College of Medicine,Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy,Baylor College of Medicine
Summary
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Um método é descrito para propagar tumores retinoblastoma humano em camundongos. Células tumorais são diretamente injetadas nos olhos de ratos imune deficiente. Tumores secundários foram estabelecidas com sucesso usando as duas células diretamente colhidas a partir de tumores humanos e tumorspheres cultivadas.
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Bond, W. S., Wadhwa, L., Perlaky, L., Penland, R. L., Hurwitz, M. Y., Hurwitz, R. L., Chèvez-Barrios, P. Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice. J. Vis. Exp. (54), e2644, doi:10.3791/2644 (2011).
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