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Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
JoVE Journal
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Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

DOI:
10.3791/2644-v

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07:55 min

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August 04, 2011

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, , , , , ,
1Interdepartmental Program in Translational Biology & Molecular Medicine,Baylor Tıp Fakültesi, 2Texas Children's Cancer Center,Baylor Tıp Fakültesi, 3Pediatri Anabilim Dalı,Baylor Tıp Fakültesi, 4Patoloji Bölümü,The Methodist Hospital Research Institute, 5Department of Ophthalmology,Retinoblastoma Center of Houston, 6Baylor Tıp Fakültesi,Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy,Baylor Tıp Fakültesi

Bölümler

  • 00:05Başlık
  • 01:39Preparation of Retinoblastoma Tumorspheres
  • 02:54Preparation of Tumor Cells and Animals before Injection
  • 04:14Injection of Tumor Cells
  • 05:32Monitoring Injected Mice and Harvesting Tumors
  • 06:28Retinoblastoma Tumors in Mice
  • 07:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.

Etiketler

EstablishmentPropagationHuman Retinoblastoma TumorsImmune Deficient MiceCultured TumorspheresCellular PhenotypesNew Tumors FormationRag2-/- Immune Deficient MiceVitreous CavityMurine EyesTumorspheres In VitroThree PassagesContinuing SourceWesley S. BondLalita Wadhwa

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