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Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
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August 04, 2011
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1Interdepartmental Program in Translational Biology & Molecular Medicine,Baylor Tıp Fakültesi, 2Texas Children's Cancer Center,Baylor Tıp Fakültesi, 3Pediatri Anabilim Dalı,Baylor Tıp Fakültesi, 4Patoloji Bölümü,The Methodist Hospital Research Institute, 5Department of Ophthalmology,Retinoblastoma Center of Houston, 6Baylor Tıp Fakültesi,Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy,Baylor Tıp Fakültesi
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A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.
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Bond, W. S., Wadhwa, L., Perlaky, L., Penland, R. L., Hurwitz, M. Y., Hurwitz, R. L., Chèvez-Barrios, P. Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice. J. Vis. Exp. (54), e2644, doi:10.3791/2644 (2011).
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