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Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
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JoVE Journal 医学
Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

DOI:
10.3791/2644-v

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07:55 min

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August 04, 2011

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, , , , , ,
1Interdepartmental Program in Translational Biology & Molecular Medicine,Baylor College of Medicine, 2Texas Children's Cancer Center,Baylor College of Medicine, 3小児科,Baylor College of Medicine, 4病理学科,The Methodist Hospital Research Institute, 5Department of Ophthalmology,Retinoblastoma Center of Houston, 6Baylor College of Medicine,Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy,Baylor College of Medicine

章

  • 00:05標題
  • 01:39Preparation of Retinoblastoma Tumorspheres
  • 02:54Preparation of Tumor Cells and Animals before Injection
  • 04:14Injection of Tumor Cells
  • 05:32Monitoring Injected Mice and Harvesting Tumors
  • 06:28Retinoblastoma Tumors in Mice
  • 07:13Conclusion

概要

自動翻訳

自動翻訳

A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.

タグ

EstablishmentPropagationHuman Retinoblastoma TumorsImmune Deficient MiceCultured TumorspheresCellular PhenotypesNew Tumors FormationRag2-/- Immune Deficient MiceVitreous CavityMurine EyesTumorspheres In VitroThree PassagesContinuing SourceWesley S. BondLalita Wadhwa

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