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Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
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09:54 min
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April 06, 2011
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Summary
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In this video, we demonstrate visualization of PKC translocation in living cells using fluorescently tagged PKCs.
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PKC TranslocationLive-imagingSf9 CellsAplysia Sensory NeuronsProtein Kinase CsCellular ProcessesClassical PKCsNovel Type I PKCsNovel Type II PKCsAtypical PKCsCa2+Diacylglycerol (DAG)Aplysia CalifornicaMemory FormationNervous System Specific PKC IsoformsLipid-activated KinasesPlasma MembraneSignal Transduction PathwaysSynaptic Plasticity
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Farah, C. A., Sossin, W. S. Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons. J. Vis. Exp. (50), e2516, doi:10.3791/2516 (2011).
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