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Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
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Neuroscience
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JoVE Journal Neuroscience
Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons

English

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11,884 Views

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09:54 min

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April 06, 2011

DOI:

10.3791/2516-v

DOI:
10.3791/2516-v

•

09:54 min
April 06, 2011

•

2 Views
,
1Neurology and Neurosurgery,McGill University

Summary

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In this video, we demonstrate visualization of PKC translocation in living cells using fluorescently tagged PKCs.

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PKC TranslocationLive-imagingSf9 CellsAplysia Sensory NeuronsProtein Kinase CsCellular ProcessesClassical PKCsNovel Type I PKCsNovel Type II PKCsAtypical PKCsCa2+Diacylglycerol (DAG)Aplysia CalifornicaMemory FormationNervous System Specific PKC IsoformsLipid-activated KinasesPlasma MembraneSignal Transduction PathwaysSynaptic Plasticity

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Cite this Article

Farah, C. A., Sossin, W. S. Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons. J. Vis. Exp. (50), e2516, doi:10.3791/2516 (2011).

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