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C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays
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March 11, 2011
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Here we describe methods to test C. elegans associative learning and short- and long-term associative memory. These population assays employ the worms abilities to chemotax toward volatile odorants, and form positive associations upon pairing food with the chemoattractant butanone. Increasing the number of conditioning periods induces long-term memory.
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C. ElegansPositive Butanone LearningShort-term MemoryLong-term MemoryAssociative Memory AssaysAversive Olfactory Learning ParadigmAWC Neuron-sensed Volatile Chemoattractant ButanoneChemotactic IndexPopulation Chemotaxis AssayLearning IndexFood And Butanone PairingMassed TrainingSpaced TrainingCAMP Response Element
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Kauffman, A., Parsons, L., Stein, G., Wills, A., Kaletsky, R., Murphy, C. C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays. J. Vis. Exp. (49), e2490, doi:10.3791/2490 (2011).
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