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Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster
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January 14, 2011
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The Giant Fiber System is a simple neuronal circuit of adult Drosophila melanogaster containing the largest neurons in the fly. We describe the protocol for monitoring synaptic transmission through this pathway by recording post synaptic potentials in dorsal longitudinal (DLM) and tergotrochanteral (TTM) muscles following direct stimulation of the Giant Fiber interneurons.
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Electrophysiological RecordingsGiant Fiber PathwayD. MelanogasterStartle ResponseNeuronal CircuitGiant Fiber System (GFS)Giant Fiber InterneuronsAxonsThoracic GanglionCervical ConnectiveMesothoracic Neuromere (T2)Ventral GangliaElectro-chemical SynapsesMedial DendriteIpsilateral Motorneuron (TTMn)Tergotrochanteral Muscle (TTM)Mesothoracic Femur/legPeripherally Synapsing Interneuron (PSI)Cholinergic SynapsesMotorneurons (DLMns)Dorsal Longitudinal Muscles (DLMs)Wing DepressorsDorsovental Muscles (DVMs)Indirect Flight Muscles
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Augustin, H., Allen, M. J., Partridge, L. Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster. J. Vis. Exp. (47), e2412, doi:10.3791/2412 (2011).
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