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Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster
Chapters
- 00:05Title
- 01:13Introduction
- 01:56Equipment and Materials
- 03:57Preparing the D. melanogaster
- 05:54Placing the Electrodes
- 09:32Stimulating and Recording
- 10:35Results: Response Latencies and Frequency of Following in the Giant Fiber Pathway
- 12:24Conclusion
The Giant Fiber System is a simple neuronal circuit of adult Drosophila melanogaster containing the largest neurons in the fly. We describe the protocol for monitoring synaptic transmission through this pathway by recording post synaptic potentials in dorsal longitudinal (DLM) and tergotrochanteral (TTM) muscles following direct stimulation of the Giant Fiber interneurons.
Tags
Electrophysiological RecordingsGiant Fiber PathwayD. MelanogasterStartle ResponseNeuronal CircuitGiant Fiber System (GFS)Giant Fiber InterneuronsAxonsThoracic GanglionCervical ConnectiveMesothoracic Neuromere (T2)Ventral GangliaElectro-chemical SynapsesMedial DendriteIpsilateral Motorneuron (TTMn)Tergotrochanteral Muscle (TTM)Mesothoracic Femur/legPeripherally Synapsing Interneuron (PSI)Cholinergic SynapsesMotorneurons (DLMns)Dorsal Longitudinal Muscles (DLMs)Wing DepressorsDorsovental Muscles (DVMs)Indirect Flight Muscles
