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Chapters
- 00:05Title
- 00:58Introduction
- 01:37FCU Negative Selection System in Entamoeba histolytica
- 03:23Determination of Selection Efficiency
- 04:38The FCU1/5-FC System: A Powerful Negative Selection Tool for E. histolytica Trophozoites
- 05:43Conclusion
Biz olumsuz bir seçim sisteminin gelişme raporu<em> E. histolytica</em> Transgenik kimerik protein ekspresyonu (FCU1) ve prodrogu 5-florositozin seçimi dayalı. FCU1 protein, maya sitozin deaminaz ve phosphoribosyltransferase urasil bir füzyon. FCU1, İfade sonuçlandı<em> E. histolytica</em> 5-florositozin karşı duyarlılık.
Tags
Negative Selectable MarkerEntamoeba HistolyticaAmebiasisGene ExpressionTransfectionPlasmidsReverse GeneticsHomologous RecombinationEpisomal Plasmid DNABackground Of Exogenous DNAGenomic Integration EventNegative Selection SystemYeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil5-fluorouridine-5′-monophosphate
