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一个负选择标记的发展阿米巴</em
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Development of a Negative Selectable Marker for Entamoeba histolytica
DOI:

06:05 min

December 12, 2010

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Chapters

  • 00:05Title
  • 00:58Introduction
  • 01:37FCU Negative Selection System in Entamoeba histolytica
  • 03:23Determination of Selection Efficiency
  • 04:38The FCU1/5-FC System: A Powerful Negative Selection Tool for E. histolytica Trophozoites
  • 05:43Conclusion

Summary

자동 번역

我们的报告负选择系统的发展<em> E。阿米巴</em>基于嵌合蛋白的转基因表达(FCU1)和前体药物5 - 氟胞嘧啶的选择。 FCU1蛋白是一种融合酵母胞嘧啶脱氨酶和尿嘧啶phosphoribosyltransferase。 FCU1表达导致增加<em> E。阿米巴</em>对5 - 氟胞嘧啶的敏感性。

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