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The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
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The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds

19,247 Views

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16:03 min

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February 15, 2011

DOI:

10.3791/2389-v

DOI:
10.3791/2389-v

•

16:03 min
February 15, 2011

•

1 Views
, , , , , , ,
1Department of Biomedical Engineering,University of Michigan, 2State Key Laboratory of Bioelectronics,Southeast University, 3Department of Neurology,University of Michigan , 4Geriatric Research, Education and Clinical Center,Veterans Affairs Ann Arbor Health System

Summary

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Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.

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Leach, M. K., Feng, Z., Gertz, C. C., Tuck, S. J., Regan, T. M., Naim, Y., Vincent, A. M., Corey, J. M. The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds. J. Vis. Exp. (48), e2389, doi:10.3791/2389 (2011).

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